CELL CULTURE LABORATORY
The Cell Culture Laboratory is a research facility where studies are conducted to propagate various cell types under controlled and sterile conditions, assess their viability, and utilize them in various experimental applications. The laboratory performs basic cell culture procedures, such as culturing cells in appropriate media, propagating them, passaging them, cryopreserving them, and preparing them for experimental studies.
Laboratory research focuses particularly on ''cancer biology, cell viability, the effects of drugs and compounds on cells, cytotoxicity, apoptosis, and cellular response mechanisms''. Using various cancer and normal cell lines, the effects of different molecules on cell proliferation and viability are investigated; MTT and similar cell viability assays, microscopic evaluations, and molecular analyses are performed.
In addition, by evaluating the biological changes resulting from exposing cells to specific agents, we investigate ''cancer cell proliferation, cell death, oxidative stress, inflammatory responses, and cellular responses to treatment''. The findings obtained form the basis for cancer research and efforts to develop new treatment approaches.
All studies conducted in the laboratory are based on ''aseptic techniques, prevention of contamination, cultivation of cells under appropriate conditions, and ensuring experimental reliability''. Within this framework, controlled experimental studies are carried out using a cell culture cabinet, a CO₂ incubator, a centrifuge, an inverted microscope, and various other laboratory equipment.
The Molecular Genetics Laboratory is a research facility where the structure, function, and cellular mechanisms of DNA, RNA, and genes are studied at the molecular level. Experimental studies are conducted in this laboratory to isolate, amplify, and analyze genetic material, as well as to evaluate gene expression.
The laboratory primarily conducts research on major genetic diseases, cancer genetics, molecular diagnostics, bioinformatics-supported gene analyses, and cellular molecular mechanisms. The studies involve the isolation of DNA and RNA from blood, tissue, and cell samples, followed by quality and quantity analyses of the obtained nucleic acids.
As part of molecular genetics research, molecular biology techniques such as the Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), gel electrophoresis, cDNA synthesis, gene expression analysis, and mutation and genetic variation analyses are used. These methods are used to examine gene expression levels, investigate genetic changes associated with diseases, and evaluate the molecular basis of biological processes.
The research conducted in the laboratory is aimed at elucidating the molecular mechanisms of cancer, identifying biomarkers, developing targeted treatment approaches, and contributing to genetic research. Sterile working conditions, proper sample preparation, the collection of high-quality data, and reliable laboratory practices are fundamental to all experimental processes.